Fig 1: Activation of SCDH microglia on PID7 following SNS at various frequencies in L5 NRL rats. Transverse sections of L5 spinal cord were obtained from NRL rats on PID7 (N = 5). Immunofluorescence staining of the microglia marker Iba1 (red) on PID7 (a). The right panels show magnification (× 10) of the ipsilateral SCDH. The relative area and integrated density of ipsilateral and contralateral Iba1 signal was quantified using ImageJ software (b, c). Scale bars = 500 µm and 200 µm (magnification). Data are expressed as mean ± SD. *p < 0.05, **p < 0.01. ***p < 0.001